Sheep Tumor Necrosis Factor, TNF

Product name

Tumor necrosis factor-alpha (TNF-alpha), ELISA kit

Full Product Name

Sheep Tumor Necrosis Factor Alpha ELISA Kit

Product synonym names

Tumor necrosis factor-alpha

Product gene name: TNF-alpha ELISA kit

Product synonym Gene name: TNF-a

Species reactivity: Sheep


This assay has high sensitivity and excellent specificity for the detection of TNF-alpha. No significant cross-reactivity or interference was observed between TNF-alpha and analogs.


Serum, plasma, tissue homogenates, and other biological fluids

Test type: Quantitative sandwich

Detection range: 15,625-1000 pg / ml

Sensitivity: <9,375 pg / ml

Intra-assay precision

Intra-assay precision (precision within an assay): 3 samples with low, medium and high levels of TNF-alpha were tested 20 times on one plate, respectively. Intra-assay: CV <8%

Interassay precision

Interassay Precision (Between Assay Precision): 3 samples were run with low, medium, and high-level TNF-alpha on 3 different plates, with 8 replicates on each plate. CV (%) = SD / mean X100. Inter-assay: CV <10%

Preparation and storage

Store at 4 degrees C if the kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, lyophilized standard, and concentrated biotinylated detection protein are stored at -20 degrees C. Other components at 2-8 degrees C). Stable for 12 months (if the entire kit is stored at -20 degrees C).

ISO certification

Manufactured in an ISO 13485: 2003 and EN ISO 13485: 2012 Certified Laboratory.

Product note

Information selected from the online data sheet is extracted from bioinformatics databases, occasionally resulting in the ambiguous or irrelevant product information. It is the customer’s responsibility to review, verify and evaluate the information to make sure it matches their requirements before purchasing the kit. The essays in our ELISA kit are dynamic research tools and can sometimes be updated and improved.

If the format of this assay is important to you, please request the current manual or contact our technical support team with a pre-sale consultation before placing an order. We will confirm the current details of the trial. We cannot guarantee that the sample manual posted online is the most current manual, it is intended to serve as an example only. See the instructions for use provided with the test kit for precise details.

Other notes

Occasionally, small volumes of TNF-alpha ELISA kit vials may become trapped in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Some products may need to be shipped with dry ice and an additional fee may apply for dry ice.

Search terms for purchasing TNF-alpha

MBS763586 is a ready-to-use microwell plate ELISA (enzyme-linked immunosorbent assay) kit for testing for the presence of tumor necrosis factor-alpha (TNF-alpha) ELISA kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls provide a theoretical detection range of the kit in biological research samples containing TNF-alpha.

The analytical biochemical ELISA technique of the MBS763586 kit is based on TNF-alpha antibody-TNF-alpha antigen interactions (immunoadsorption) and an HRP colorimetric detection system to detect TNF-alpha antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant TNF-alpha. Appropriate sample types may include secretions and undiluted body fluids and/or tissue homogenates. Quality control trials evaluating reproducibility identified the intra-assay CV (%) and inter-assay CV (%).

Related Product Information for TNF-alpha ELISA kit

Assay Principle: This kit was based on enzyme-linked immunosorbent sandwich assay technology. It was precoated with an anti-TNF-alpha antibody on 96-well plates. And biotin-conjugated anti-TNF-alpha antibody was used as detection antibodies. Standards, test samples, and biotin-conjugated detection antibodies were subsequently added to the wells and washed with wash buffer.

HRP-streptavidin was added and unbound conjugates were washed with wash buffer. TMB substrates were used to visualize the HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue product that changed to yellow after adding an acid stop solution. The density of yellow is proportional to the amount of TNF-alpha sample captured on the plate. Read the D.O. absorbance at 450 nm in a microplate reader, and then the concentration of TNF-alpha can be calculated.


All MyBioSource products are for scientific laboratory research purposes and not for diagnostic, therapeutic, prophylactic, or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Product purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order when we reasonably believe that the intended use will be outside of our acceptable guidelines.


While every effort has been made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be responsible for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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