collagen, type X, alpha 1 (COL10A1), ELISA kit
Full Product Name
Human Collagen Alpha-1 (X) Chain, ELISA Kit COL10A1
Product synonym names
Human collagen alpha-1 (X) chain ELISA kit (COL10A1); OTTHUMP00000017049; Schmid metaphyseal chondrodysplasia; collagen X; alpha-1 polypeptide; type X alpha 1 collagen; type X collagen; alpha 1
Product gene name
COL10A1 ELISA kit
OMIM: gen 156500
3D structure: ModBase 3D Structure for Q03692
Species reactivity: Human
This assay has high sensitivity and excellent specificity for the detection of human COL10A1. No significant cross-reactivity or interference was observed between human COL10A1 and analogs.
Serum, plasma, tissue homogenates, cell lysates
Test type: Sandwich
Detection range: 0.312 ng / ml-20 ng / ml
Sensitivity: 0.078 ng / ml
- Intra-assay precision (precision within an assay): CV% is less than 8%
- Three samples of known concentration were run twenty times on a plate for evaluation.
- Inter-assay precision (precision between assays): CV% is less than 10%
- Three samples of known concentration were analyzed in twenty tests for evaluation.
Detection wavelength: 450 nm
Sample volume: 50-100ul
Preparation and storage
Unopened test kits should be stored at 2-8 degrees C upon receipt. See pdf manual for further storage instructions.
Manufactured in an ISO 9001: 2015 certified laboratory.
Information selected from the online data sheet is extracted from bioinformatics databases, occasionally resulting in the ambiguous or irrelevant product information. It is the customer’s responsibility to review, verify and evaluate the information to make sure it matches their requirements before purchasing the kit. The essays in our ELISA kit are dynamic research tools and can sometimes be updated and improved.
If the format of this assay is important to you, please request the current manual or contact our technical support team with a pre-sale consultation before placing an order. We will confirm the current details of the trial. We cannot guarantee that the sample manual posted online is the most current manual, it is intended to serve as an example only. See the instructions for use provided with the test kit for precise details.
Small volumes of COL10A1 ELISA kit vial (s) may occasionally get caught in the product vial seal during shipping and storage. If necessary, briefly centrifuge the vial in a benchtop centrifuge to dislodge any liquid on the lid of the container. Some products may need to be shipped with dry ice and an additional fee may apply for dry ice.
Search terms for purchasing COL10A1
MBS917063 is a ready-to-use microwell plate ELISA (enzyme-linked immunosorbent assay) kit for testing for the presence of target analytes of the collagen, type X, alpha 1 (COL10A1) ELISA kit in biological samples. The concentration gradients of the kit standards or positive controls give a theoretical detection range of the kit in biological research samples containing COL10A1.
The analytical biochemical ELISA technique of the MBS917063 kit is based on COL10A1 antibody-COL10A1 antigen interactions (immunosorbent) and an HRP colorimetric detection system to detect COL10A1 antigen targets in samples. The ELISA Kit is designed to detect native, non-recombinant COL10A1. Appropriate sample types may include secretions and undiluted body fluids and/or tissue homogenates. Quality control trials evaluating reproducibility identified the intra-assay CV (%) and inter-assay CV (%).
Related Product Information for COL10A1 kit ELISA
Assay Principle: This assay employs the quantitative sandwich enzyme immunoassay technique. A microplate has been pre-coated with an antibody specific for COL10A1. Standards and samples are pipetted into the wells and any COL10A1 present binds to the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL10A1 is added to the wells.
After washing, avidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and the color develops in proportion to the amount of COL10A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.